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Immunohistochemistry (Frozen sections) - Mounting Medium With DAPI - Aqueous, Fluoroshield (ab104139)
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI (ab104139).
This image is courtesy of a customer review submitted by Bryan Niedenberger.

Immunocytochemistry/ Immunofluorescence - Mounting Medium With DAPI - Aqueous, Fluoroshield (ab104139)
ab104139 worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus.
This image is courtesy of a customer review submitted by Sebastian Alvarado.

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