Mounting Medium With DAPI - Aqueous, Fluoroshield(AB104139)
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Mounting Medium with DAPI ab104139 (previously called Fluoroshield Mounting Medium With DAPI) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. Suitable to prevent rapid photobleaching for IHC, ISH and ICC/IF applications.
- Proven performance: cited in over 325 publications
- Compatible with our range of fluorescent labeled secondary antibodies
- Available in different sizes to fit your experimental needs
Product Detail - Mounting Medium with DAPI - Aqueous, Fluoroshield (AB104139)
Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC.
This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
Immunohistochemistry (Frozen sections) - Mounting Medium with DAPI - Aqueous, Fluoroshield (ab104139)
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI (ab104139).
This image is courtesy of a customer review submitted by Bryan Niedenberger.
Immunocytochemistry/ Immunofluorescence - Mounting Medium with DAPI - Aqueous, Fluoroshield (ab104139)
ab104139 worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus.
This image is courtesy of a customer review submitted by Sebastian Alvarado.
| Shipped At Conditions | Blue Ice |
| Appropriate Long term Storage Conditions | Store at +4°C. |
| Applications | ICC/IF, IHC-Fr, IHC-P, ISH |