Our antibody arrays are designed to streamline research efforts by allowing broad examination of relevant targets, saving research time and reducing costs. Our antibodies offer exceptional sensitivity and dynamic ranges, making them suitable for applications such as inflammation research, biomarker profiling, and disease monitoring. Available in both quantitative and membrane-based formats, these arrays can be used to process multiple samples in parallel, significantly reducing the time, complexity, and cost associated with traditional methods like ELISA.
FEATURES
- Specially curated antibody panels and arrays for specific signaling pathways or mechanisms.
- Comprehensive profiling of related proteins across human, mouse, and rat samples.
- Quantitative arrays with a broad dynamic range.
- Rigorous testing to prevent false positives by evaluating antibody pair interactions.
- Membrane-based arrays enable simultaneous measurement of up to 96 proteins.
- Cost-effective, reducing expenses by approximately 50% per analyte.
- No specialized equipment is required beyond standard chemiluminescent western blotting tools.
- Optimized kits include all necessary components, minimizing variability between assays.
APPLICATIONS
- Cytokine expression profiling: Analyze cytokine levels in various samples.
- Serum biomarker profiling: Identify and quantify biomarkers in serum.
- Confirmation of PCR, gene microarray, or western blotting results: Validate your results obtained from other techniques.
- Disease research: Identify active biological processes in diseases such as asthma, cancer, and autoimmune disorders.
- Relative profiling: Compare protein levels in normal, diseased, and treated samples.
FAQs
What is an antibody array, and how does it function?
An antibody array is a high-throughput platform designed to detect and quantify multiple proteins simultaneously. It operates similarly to an ELISA but allows for the analysis of numerous proteins in one experiment by fixing capture antibodies on a solid surface.
What should be considered when choosing an antibody array for a study?
When choosing an antibody array for a study, important considerations include the number and type of target proteins, sample volume requirements, detection method (chemiluminescence or fluorescence), level of quantification (qualitative, semi-quantitative, or fully quantitative), compatibility with existing equipment, and the dynamic range necessary for accurate detection.
How can sample preparation for antibody array experiments be optimized?
To optimize the sample preparation procedure for antibody array experiments, ensure proper collection, storage, and processing to maintain protein integrity. Use suitable lysis buffers, remove debris through centrifugation, normalize protein concentrations, perform as many steps on ice as possible, and avoid freeze-thaw cycles to ensure consistent and reliable results.
What are the differences between membrane-based and glass-slide antibody arrays?
The differences between membrane-based and glass-slide antibody arrays include the detection method and equipment requirements. Membrane-based arrays use chemiluminescent detection and can be analyzed with standard western blot equipment, making them cost-effective and user-friendly. In contrast, glass slide arrays rely on fluorescence detection, require a laser slide scanner, and offer a broader dynamic range and quantitative analysis.
Can antibody arrays be used for multiplex protein detection and quantification?
Yes, antibody arrays can be used for multiplex protein detection and quantification, enabling the simultaneous measurement of multiple proteins in a single experiment.