Alexa Fluor® Secondary Antibodies
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Our Alexa Fluor®-conjugated secondary antibodies deliver bright staining with minimal background, using Alexa Fluor® dyes for unmatched fluorescence intensity and minimal spectral overlap.
Our Alexa Fluor® secondary antibodies are labeled with highly fluorescent and photostable Alexa Fluor® dyes. These antibodies are ideal for detecting specific IgG subclasses, allowing for the identification of multiple targets. To reduce background caused by cross-species reactivity, Alexa Fluor® pre-adsorbed secondary antibodies are recommended. Each product undergoes rigorous in-house testing to ensure exceptional performance in applications such as cell imaging, flow cytometry, and fluorescent western blotting. Our range includes  secondary antibodies conjugated with Alexa Fluor® 405, 488, 555, 568, 594, 647, 680, 750, and 790 dyes, each offering distinct fluorescence properties for various research needs. These antibodies are validated with multiple biological samples, ensuring consistent performance across different sample types.
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Features

  • Superior brightness:  Excellent fluorescence intensity.
  • Exceptional photostability:  Enables extended visualization and high-quality image capture.
  • pH resilience: Maintains strong fluorescence across a broad pH range.
  • Enhanced water solubility:  Prevents antibody precipitation and aggregation.
  • Optimized quality:  Calibrated dye-to-antibody ratio reduces photobleaching.
  • Low background staining:  Rigorous column separation process for cleaner staining.

Applications

  • Flow cytometry (Flow Cyt):  Facilitates the detection and analysis of cell surface and intracellular proteins in suspension.
  • Immunocytochemistry/immunofluorescence (ICC/IF):  Ideal for visualizing protein localization in cultured cells using fluorescence microscopy.
  • Fluorescence western blotting:  Provides high sensitivity for detecting target proteins with minimal background.
  • Live-cell imaging:  Suitable for tracking proteins and other targets in living cells without compromising cell viability.
  • Validated for use with various sample types:  Ensures consistent performance across different biological samples.
FAQs

What is Alexa Fluor® used for?

Alexa Fluor® dyes are used for fluorescence labeling in various biological applications, including  flow cytometry, immunohistochemistry, and  microscopy, for high-sensitivity detection of specific proteins and biomolecules.

What are the common applications of Alexa Fluor® secondary antibodies?

Alexa Fluor® secondary antibodies are widely used in immunofluorescence techniques such as immunocytochemistry (ICC), flow cytometry, and ELISA, providing high brightness and minimal background fluorescence.

Can Alexa Fluor® secondary antibodies be used for live-cell imaging?

Yes, the excellent photostability and minimal background make Alexa Fluor® secondary antibodies suitable for tracking proteins and other targets in living cells.

Are Alexa Fluor® secondary antibodies suitable for super-resolution microscopy?

Yes, the high brightness and photostability of Alexa Fluor® secondary antibodies allow for precise imaging at high resolution, which is ideal for techniques like STORM and PALM.

Can Alexa Fluor® secondary antibodies be used in western blotting?

Yes, Alexa Fluor® secondary antibodies provide high sensitivity for detecting target proteins with minimal background, making them excellent for fluorescence-based western blot detection.

Can Alexa Fluor® secondary antibodies be used in combination with other fluorescent dyes?

Yes, Alexa Fluor® secondary antibodies can be combined with other fluorescent dyes for multiplexing techniques, ensuring minimal spectral overlap.