Abcam is dedicated to meeting the evolving needs of the scientific community through its expansive immunoassay solutions. Our solutions are designed to deliver unparalleled precision and accuracy for protein detection, ensuring reliability across a variety of applications.
The Simple Step ELISA® kits in our portfolio offer a rapid, single-wash protocol for streamlined workflows, significantly reducing assay time without compromising data quality. Similarly, our multiplex kits allow for the simultaneous detection of multiple analytes, enhancing efficiency and throughput in complex studies. This capability makes them indispensable for researchers aiming to maximize productivity. Our matched antibody pair kits consist of carefully selected capture and detection antibodies, enabling researchers to create custom sandwich assays with high specificity and reproducibility.
In addition to assays, our range of antibodies is unmatched in diversity. With products covering antigens from multiple species, such as humans, mice, and rats, researchers have access to a versatile toolkit. Our recombinant monoclonal antibody technology ensures batch-to-batch consistency and high specificity. Antibodies are available with a choice of tags, including biotin, FITC, PE, metals, and HRP, catering to various research requirements.
Beyond immunoassays, our diverse antibody portfolio supports applications such as western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, and chromatin immunoprecipitation (ChIP). Our products are extensively validated, including knockout validation, to ensure their reliability in experimental settings. Our carrier-free formulations allow for custom conjugation with the user’s choice of labels, further enhancing flexibility.
Abcam prioritizes quality, reproducibility, and user convenience, empowering researchers to advance science through our reliable and innovative immunoassay tools.
Features
- Extensive antibody collection: A comprehensive collection of antibodies targeting antigens from various species, including humans, mice, and rats, to support diverse research applications.
- Recombinant monoclonal technology: Ensuring consistent specificity and reproducibility across various experimental settings, as well as scalability.
- Multiple tag options: Including biotin, FITC, PE, HRP, and metals.
- Species variety: Antibodies raised in various species, such as rabbits, mice, goats, and rats, ensuring versatility for various research models.
- Comprehensive validation: Guaranteeing accuracy and reliability in experimental outcomes.
- Carrier-free formulations: Carrier-free formulations are adaptable for custom conjugation, giving researchers flexibility in tailoring antibodies.
- A range of isotypes: A variety of antibody isotypes are available, allowing you to match your specific experimental requirements.
- Trial sizes: Trial sizes of select antibodies are provided for preliminary testing to optimize research processes efficiently.
Applications
- Western blot: Enables precise detection and analysis of proteins in sample materials.
- Immunocytochemistry (ICC) and immunohistochemistry (IHC): Detect and analyze protein expression in cells and tissues.
- Flow cytometry: Analyze cell populations, protein expression, and cellular characteristics.
- Multiplex IHC: Detect multiple targets simultaneously within a single tissue sample.
- Chromatin immunoprecipitation (ChIP): Study protein–DNA interactions to uncover regulatory mechanisms.
- Multiplex assays: Efficiently detect and quantify multiple analytes in a single experiment.
What are immunoassays?
Immunoassays are biochemical tests that detect and measure specific molecules, such as proteins or hormones, within a sample. They rely on the precise binding between antibodies and their target antigens to ensure accurate results. Commonly used in diagnostics and research, immunoassays are pivotal for studying biological processes and disease mechanisms.
How are matched antibody pair kits utilized in research?
Matched antibody pair kits contain two antibodies that work together to capture and detect a target molecule in sandwich assays. These matched antibody pairs are validated to bind to different epitopes on the same antigen. The capture antibody binds to the analyte, while the detection antibody ensures specificity and measurable output. These kits simplify assay development and enhance the reproducibility of experimental outcomes.
What are multiplex kits?
Multiplex kits enable researchers to measure multiple analytes simultaneously in a single experiment. Utilizing technologies such as bead arrays or microarrays, these kits enable high-throughput and efficient analysis. They are ideal for studies requiring comprehensive data, such as cytokine profiling or biomarker discovery.
How are antibodies used in western blot, ICC, IHC, and flow cytometry?
In western blot, antibodies detect specific proteins in a mixture after separation by size, aiding in understanding protein expression and modification. ICC and IHC utilize antibodies to visualize protein localization in cells or tissues, offering insights into biological functions. In flow cytometry, fluorescently tagged antibodies label proteins on cells, enabling population analysis and functional studies.
What is an immunoassay kit used for?
An immunoassay kit is used to detect and measure specific molecules, such as proteins, hormones, or antibodies, in a biological or chemical sample. These kits rely on antigen-antibody interactions to provide accurate and sensitive measurements, making them valuable tools in diagnostics, biomedical research, and pharmaceutical development. Common applications include identifying biomarkers, studying disease mechanisms, monitoring therapeutic responses, and analyzing complex biological processes.
What are the key components of an immunoassay?
An immunoassay consists of several essential components. Antibodies play a key role, as they are proteins specifically designed to bind to the target molecule (antigen) with high precision. Antigens, which are the molecules being detected or measured, include proteins, hormones, or other analytes present in the sample.
The detection system is another critical component, often involving labels such as enzymes, fluorescent dyes, or radioactive isotopes that produce measurable signals. Solid-phase supports, like microtiter plates or beads, provide a surface where the immunoassay reactions occur, facilitating separation and signal generation. Blocking agents are also included to prevent nonspecific binding, ensuring the accuracy and reliability of the assay.