Our range of carrier-free antibodies is optimized for efficient antibody labeling, streamlining the process with an easy-to-follow protocol. They can be directly added to the conjugation reaction, eliminating the need for buffer exchange or concentration steps and minimizing antibody loss.
Our carrier-free antibodies are free from additives commonly found in standard rabbit monoclonal antibodies, such as bovine serum albumin (BSA), sodium azide, and glycerol. These components often impede effective conjugation to fluorochromes, metals, oligonucleotides, and enzymes, reducing labeling efficiency. BSA, for example, competes with the primary antibody for labeling, while sodium azide can inhibit horseradish peroxidase activity and exert a toxic effect on cells. Removing these additives enhances conjugation efficiency and broadens the applications of the antibodies.
Low endotoxin, azide-free bioactive antibodies address the challenges posed by endotoxins, heat-stable contaminants that are difficult to eliminate through standard filtering or autoclaving. Designed for a variety of applications, our carrier-free antibodies feature low endotoxin levels (≤1 EU/ml). These antibodies are supplied at a high concentration of 1 mg/mL for ease of use and undergo our extensive validation and testing in a range of applications and for specificity. They are available in bulk sizes and customizable concentrations, providing flexibility for your experimental needs.
The use of purified IgG ensures higher conjugation efficiency by eliminating contaminants such as stabilizers, proteins, or preservatives that may interfere with the labeling process. This results in a more stable and reproducible conjugation reaction, improving signal strength and assay sensitivity. By using purified IgG, researchers can achieve optimal performance in applications such as flow cytometry, immunofluorescence, and ELISA, where precise and consistent antibody labeling is critical for accurate data interpretation.
Our carrier-free antibodies give researchers the flexibility to select their own conjugates. This allows customization for specific experimental needs. As a result, researchers can expand the range of experiments they perform.
Moreover, combining carrier-free antibodies with our Lightning-Link® kits simplifies the conjugation process. This approach eliminates complex labeling steps. As a result, researchers can achieve fast and efficient antibody conjugation.
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Features
- Carrier-free antibodies are specifically designed to streamline labeling processes, while carrier-free matched antibody pairs offer flexibility and customization for various assays.
- These products are formulated without BSA, sodium azide, or glycerol, ensuring consistent and efficient conjugation.
- The antibodies are ready for use with our Lightning-Link® kits.
- The carrier-free formulations are compatible with a wide range of labels, including fluorochromes, metal isotopes, oligonucleotides, and enzymes, making them suitable for diverse applications.
- The use of purified IgG further enhances conjugation yield, providing optimal performance.
- Recombinantly manufactured, our carrier-free antibodies provide exceptional batch-to-batch consistency, ensuring reliable results across experiments.
- Supplied at a high concentration of 1 mg/mL, we eliminate the need for additional concentration steps.
- Designed for seamless integration, these carrier-free products offer flexibility and efficiency in experimental workflows.
Applications
- Carrier-free antibody samples can be used for flow-based techniques that analyze multiple parameters simultaneously at the single-cell level.
- Carrier-free antibodies are highly effective in multiplex IHC assays, supporting advanced imaging techniques that combine metal-conjugated antibodies with immunohistochemical methods to study cell-cell interactions at subcellular resolution and analyze tumor heterogeneity. Additionally, they enable cyclic immunofluorescence approaches, which involve iterative staining and imaging, providing a cost-effective and reliable solution for multiplex imaging.
- Carrier-free antibodies are ideal for ELISA-based assays, functional assays, and high-throughput screening due to their BSA, azide, and glycerol-free formulation.
- Over 4,000 matched antibody pairs are available in carrier-free formats, which are compatible with assays beyond ELISA, including fluorescent proximity assays like Time-Resolved Fluorescence Energy Transfer (TR-FRET).
Why are carrier-free antibodies important?
Carrier-free antibodies are important because they eliminate additives like BSA, sodium azide, and glycerol, which can interfere with labeling and conjugation processes. This ensures higher conjugation efficiency and avoids the need for buffer exchange or concentration steps, saving time and reducing antibody loss.
Can carrier-free antibodies be used in in vivo studies?
Yes, carrier-free antibodies can be used in in vivo studies. Sodium azide is toxic to cells and thus not ideal for live applications; our carrier-free antibody range is formulated to be free of these additives. Their low endotoxin levels make them ideal for applications such as neutralization, blocking, and activation studies in live animal models. Additionally, the absence of carrier proteins like BSA ensures they do not interfere with biological systems, making them highly reliable for in vivo research.
How do carrier-free antibodies improve conjugation workflows?
Carrier-free antibodies improve conjugation workflows by eliminating additives such as BSA, sodium azide, and glycerol, which can interfere with labeling efficiency. Without these carriers, antibodies can be directly added to conjugation reactions without the need for buffer exchange or concentration steps, reducing handling time and minimizing material loss. This streamlined process ensures consistent and efficient conjugation with a wide variety of labels, such as fluorochromes, metals, enzymes, and oligonucleotides.
How are carrier-free antibodies prepared?
Carrier-free antibodies are prepared by removing additives from standard antibody formulations, typically through purification techniques like dialysis or chromatography. These processes ensure the antibodies are in a pure, buffer-only solution, making them suitable for direct conjugation and various downstream applications.