Modern drug development relies on generating high-quality, actionable data at every stage of the process. As therapeutics grow more complex, analytical technologies have become crucial for understanding molecular behavior, ensuring product quality and meeting evolving regulatory standards.

Flow cytometry platforms provide critical insights into cell identity, viability and function, making them especially valuable for genomic medicines such as cell and gene therapies.

This enabling technology is invaluable across many fields and serves as an essential component of the analytical toolbox for virtually every current and emerging therapeutic modality. One crucial factor to consider before leveraging flow cytometry is the size of your panel. For larger panels, having a properly fitted instrument that can distinguish emission overlap from your fluorochromes or dyes is critical.

Spectral flow cytometry offers a significant advantage by resolving fluorochromes with similar emission maxima but distinct off-peak spectra, thereby allowing for the inclusion of a greater variety of fluorochromes in a single experiment.

Flow cytometry can perform the following:

Key Applications of Flow Cytometry

• Monoclonal Antibodies – Monitors the receptor expression levels to validate target engagement
• CAR-T Cell Therapies - Quantifies tumor cell killing and cytokine production in T cells to evaluate efficacy and safety
• mRNA Vaccines - Monitors protein expression levels and innate immune activation to assess risk of inflammatory responses
• Gene Editing - Evaluates editing efficiency and off-target effects via surface and intracellular markers
• Gene Therapy - Identifies unintended transduction in non-target cell types to evaluate biodistribution and safety

Flow cytometry without limits: Beckman Coulter Life Sciences has expanded the analytical toolkit with innovative flow cytometry solutions that anticipate the future of your science.

Flow cytometry without limits: Beckman Coulter Life Sciences has expanded the analytical toolkit with innovative flow cytometry solutions that anticipate the future of your science.

CytoFLEX nano Flow Cytometer

The CytoFLEX nano Flow Cytometer the newest member of the CytoFLEX Platform. Specifically designed for the analysis of nanoparticles, it gives researchers a sensitive, consistent and flexible solution that can help them analyze nanoparticles as small as 40 nm.

This innovative solution can be used to characterize and phenotype, including the packaging efficiency of:

• Extracellular vesicles (EVs)
• Lipid nanoparticles (LNPs)
• Liposomes
• Viral vectors (AAVs, LVs)
• Virus-like particles (VLPs)

CytoFLEX mosaic Spectral Detection Module

The CytoFLEX mosaic spectral detection module represents the next step in the evolution of our CytoFLEX platform. It provides spectral flow cytometry capabilities for our CytoFLEX S and LX instruments. With this module, you can mix and match different detection methods in one instrument, increasing capacity without the need to invest in a new independent instrument.

We understand that resources and sustainability are critical considerations. That’s why we’ve introduced spectral technology as a modular detection concept, allowing you to enhance your capabilities while future-proofing your existing instruments.

As new therapies emerge, analytical needs will continue to evolve. Our technologies are designed to meet today’s challenges and scale with tomorrow’s pipelines. Contact an expert today to learn how we can partner with you to address your analytical needs.