CytoFLEX nano Flow Cytometer
The CytoFLEX nano Flow Cytometer is the newest member of the CytoFLEX Platform.
Specifically designed for the analysis of nanoparticles, the CytoFLEX nano Flow Cytometer overcomes the limitations of traditional flow cytometry, giving researchers a sensitive, consistent and flexible solution that can help them advance their research.
The CytoFLEX nano Flow Cytometer is a pivotal development in flow cytometry that allows users to analyze extracellular vesicles (EVs) at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection and 5 side scatter channels, to deliver full characterization and open additional avenues for your nanoparticle research.
CytoFLEX nano Flow Cytometer Features
Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.
Sensitivity
With an unparalleled size sensitivity from 1µm to 40nm* and 10nm resolution,** the CytoFLEX nano flow cytometer enables the detection of smaller EV populations and their low-abundance cargo.
Flexibility
Open up your research with 6 fluorescent channels and 5 side scatter channels. Gather all the necessary data with fewer constraints.
Maximum Consistency
Maintaining contamination-free, robust instrument performance is critical when working with small size particles. Obtain reproducible results you can trust with >90% volumetric counting accuracy and <1% carryover between samples thanks to automated QC controls.
Familiarity
Built on the same principles as the CytoFLEX platform, the CytoFLEX nano flow cytometer features a similar footprint and user-friendly software interface.
| Violet side scatter sensitivity | VSSC1: 40 nm relative to polystyrene nanoparticles VSSC2: 80 nm relative to polystyrene nanoparticles |
| Scatter detection dynamic range | VSSC 1 for small range 40-150 nm polystyrene nanoparticles VSSC2 for large range 80-1000 nm polystyrene nanoparticles |
| Violet forward scatter sensitivity | 300 nm relative to polystyrene nanoparticles |
| Fluorescence sensitivity and resolution | Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity FluorospheresV477: 8 peaksB531: 8 peaksY595: 8 peaksR670: 6 peaksR710: 5 peaksR792: 4 peaks |
| Fluorescence rCV | CV < 10% (using QC Fluorospheres at 1 µL/min) |
| Carryover between Samples | ≤1%a |
| Acquisition speed (Maximum electronic acquisition speed) | 16000 events/second with ≤ 95% yield |
| Acquisition speed (Recommended maximum sample acquisition speed) | 5000 events/second to avoid possible swarming or coincidence situations. |
| Volumetric Counting accuracy | > 90%b |