CytoFLEX nano Flow Cytometer

The CytoFLEX nano Flow Cytometer is the newest member of the CytoFLEX Platform. Specifically designed for the analysis of nanoparticles, the CytoFLEX nano Flow Cytometer overcomes the limitations of traditional flow cytometry, giving researchers a sensitive, consistent and flexible solution that can help them advance their research. The CytoFLEX nano Flow Cytometer is a pivotal development in flow cytometry that allows users to analyze extracellular vesicles (EVs) at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection and 5 side scatter channels, to deliver full characterization and open additional avenues for your nanoparticle research.

Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.

Sensitivity

With an unparalleled size sensitivity from 1µm to 40nm* and 10nm resolution,** the CytoFLEX nano flow cytometer enables the detection of smaller EV populations and their low-abundance cargo.

Flexibility

Open up your research with 6 fluorescent channels and 5 side scatter channels. Gather all the necessary data with fewer constraints.

Maximum Consistency

Maintaining contamination-free, robust instrument performance is critical when working with small size particles. Obtain reproducible results you can trust with >90% volumetric counting accuracy and <1% carryover between samples thanks to automated QC controls.

Familiarity

Built on the same principles as the CytoFLEX platform, the CytoFLEX nano flow cytometer features a similar footprint and user-friendly software interface.

Specifications
Violet side scatter sensitivity	VSSC1: 40 nm relative to polystyrene nanoparticles VSSC2: 80 nm relative to polystyrene nanoparticles
Scatter detection dynamic range	VSSC 1 for small range 40-150 nm polystyrene nanoparticles VSSC2 for large range 80-1000 nm polystyrene nanoparticles
Violet forward scatter sensitivity	300 nm relative to polystyrene nanoparticles
Fluorescence sensitivity and resolution	Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity FluorospheresV477: 8 peaksB531: 8 peaksY595: 8 peaksR670: 6 peaksR710: 5 peaksR792: 4 peaks
Fluorescence rCV	CV < 10% (using QC Fluorospheres at 1 µL/min)
Carryover between Samples	≤1%a
Acquisition speed (Maximum electronic acquisition speed)	16000 events/second with ≤ 95% yield
Acquisition speed (Recommended maximum sample acquisition speed)	5000 events/second to avoid possible swarming or coincidence situations.
Volumetric Counting accuracy	> 90%b