Optimizing Biomarker Analysis for Extracellular Vesicles and Tissue Samples

Studying biomarkers can help researchers to develop targeted therapies for various illnesses, including cancer. Extracellular vesicles (EVs) are a rich source of information on human-relevant biomarkers and disease states since they are secreted by almost all cell types, allowing for noninvasive sampling called “liquid biopsies.” Additionally, tissue samples are vital for understanding in vivo disease states. However, analyzing EVs and tissue-based biomarkers remains challenging due to their variable sizes, the requirement for robust sensitivity, the need to manage large datasets, and the absence of standardized analytical methodologies.

EVs are often too small to be accurately measured by conventional flow cytometry instruments. However, flow cytometers, such as Beckman Coulter Life Sciences CytoFLEX nano Flow Cytometer, are able to characterize smaller populations of EVs and provide researchers with critical information on vesicle sizes and payloads. The Beckman Coulter Life Sciences CytoFLEX nano Flow Cytometer also offers flexibility and consistency for highly accurate counting results, even for small vesicle species.

When performing molecular analysis, the quantitation and identification of seemingly similar biomarkers presents challenges, as it is often hard to determine which biomarkers are most relevant. User-friendly molecular characterization solutions like the SCIEX ZenoTOF 7600 System can help researchers overcome this challenge. Integrated ChemSpider and SCIEX OS software tools further enable streamlined biomarker quantification and efficient data processing.

Biomarkers promise highly personalized and effective therapies for a range of illnesses. State-of-the-art diagnostic tools will be pivotal in accelerating biomarker research. Contact an expert at the Life Sciences companies of Danaher today to explore the right tools for your lab.