Rethinking Oligonucleotide LC-MS Analysis: Optimizing Workflows for Quantification and Qualification

Oligonucleotide LC-MS workflows are often dictated by stringent project requirements, necessitating complex sample preparation to meet sensitivity demands and/or extensive chromatographic separation for ample baseline resolution. As interest in therapeutic oligos increases, teams need accessible workflows that can support quantitative and qualitative performance while enabling deeper assessment of related species, impurities, and modifications.

In this webinar, scientists from QPS will share an oligonucleotide bioanalysis case study showing how sample extraction and LC workflow decisions shaped method performance on the SCIEX ZenoTOF 8600 system.

Building on the QPS study, SCIEX showcase complementary, qualitative oligo mapping and impurity analysis using IP-RP LC-MS/MS. The discussion will demonstrate how accurate mass measurement, sequence analysis, chromatographic separation and automated data analysis can support mass analysis, impurity assessment, and sequence confirmation in addition to quantitative measurement.

By the end of this webinar, attendees will be able to:

Evaluate key oligonucleotide bioanalysis workflow to assess recovery, quantitation and study-specific challenges
Apply extraction, LC conditions and MS optimization strategies to improve quantitative results
Assess where high-resolution MS and EAD can extend bioanalysis beyond quantitation to support broader characterization