ATTO 678H Azide Hydrophilic Fluorescent Label 5 mg
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Product No. AD678H105
Like ATTO 643 and ATTO 647N, ATTO 678H belongs to a new generation of long-wavelength fluorescent markers. The dye's characteristic properties include strong absorption, good fluorescence quantum yield, and high thermal and photochemical stability.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 678 nm
- ε max = 1.5×105 M -1 cm -1
- λ fl = 708 nm
- n fl = 20 %
- τ fl = 1.5 ns
- CF 260 = ε 260 /ε max = 0.12
- CF 280 = ε 280 / ε max = 0.08
Like ATTO 643 and ATTO 647N, ATTO 678H belongs to a new generation of long-wavelength fluorescent markers. The dye's characteristic properties include strong absorption, good fluorescence quantum yield, and high thermal and photochemical stability. ATTO 678H is highly hydrophilic and highly water-soluble, and compared to ATTO 680, it exhibits significantly reduced aggregation behavior and a low tendency toward nonspecific binding. After coupling to a substrate, the dye is singly negatively charged. Unlike ATTO 680, it is not an electron acceptor; its fluorescence is not quenched by guanine, tryptophan, etc. Efficient fluorescence excitation occurs in the 665–695 nm range.
| Modification | Azide |
| Unit of Measure | 5 mg |
| Product Group | ATTO 678 H |
| Product Type | Hydrophilic Fluorescent Label |
| Fluorescence Maximum (nm) | 708 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.12 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.08 |
| Longest Wavelength Absorption Maximum (nm) | 678 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 150000 |
| Fluorescence Quantum Yield (%) | 20 |
| Real Fluorescence Decay Time (ns) | 1.5 |