ATTO 589H Carboxy Hydrophilic Fluorescent Label 5 mg

Optical properties

• λ _abs = 589 nm
• ε _max = 1.2×10⁵ M ^-1 cm ^-1
• λ _fl = 620 nm
• n _fl = 85%
• τ _fl = 3.9 ns
• CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.36
• CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.52

ATTO 589H is a fluorescent marker from the class of rhodamines. As a hydrophilic alternative to ATTO Rho101, the dye shows very good water solubility, significantly reduced aggregation behavior and a low tendency to non-specific binding. ATTO 589H has a strong absorption, very high fluorescence quantum yield and a high thermal and photochemical stability. The dye is therefore perfectly suited for use in single molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 589H is an anionic dye. After coupling to a substrate, it carries a single negative charge. Efficient excitation of fluorescence occurs in the 570 - 600 nm range. ATTO 589H is an isomer mixture of two dyes with practically identical absorption and fluorescence properties. The dye is also available in pure isomer form on request.