ATTO 576H Azide Hydrophilic Fluorescent Label 1 mg
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Product No. AD576H101
ATTO 576H is a fluorescent marker from the class of rhodamines. As a hydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 576 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 598 nm
- n fl = 90 %
- τ fl = 4.0 ns
- CF 260 = ε 260 /ε max = 0.26
- CF 280 = ε 280 / ε max = 0.10
ATTO 576H is a fluorescent marker from the class of rhodamines. As ahydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding. ATTO 576H possesses strong absorption, a very high fluorescence quantum yield, and high thermal and photochemical stability. The dye is therefore ideal for use in single-molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 576H is an anionic dye. After coupling to a substrate, it is singly negatively charged. ATTO 576H is a mixture of three isomers with virtually identical absorption and fluorescence properties. Efficient fluorescence excitation occurs in the 550–585 nm range.
| Modification | Azide |
| Unit of Measure | 1 mg |
| Product Group | ATTO 576 H |
| Product Type | Hydrophilic Fluorescent Label |
| Fluorescence Maximum (nm) | 598 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.26 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 576 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 4 |