ATTO 386H Azide Hydrophilic Fluorescent Label 5 mg
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ATTO 386H is a hydrophilic short wavelength fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 386 nm
- ε max = 2.5×104 M -1 cm -1
- λ fl = 472 nm
- n fl = 90 %
- τ fl = 5.0 ns
- CF 260 = ε 260 /ε max = 0.45
- CF 280 = ε 280 / ε max = 0.08
ATTO 386H is a hydrophilic fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, a large Stokes shift, high photostability, and a low molecular weight. As a hydrophilic variant of ATTO 390, ATTO 386H is highly water-soluble, and its fluorescence efficiency is only slightly reduced after coupling to biomolecules, such as proteins, even at higher degrees of labeling (DOL). ATTO 386H is an anionic dye. After conjugation to a biomolecule, the dye carries a singly negative charge. Efficient fluorescence excitation occurs in the 365–410 nm range.
| Modification | Azide |
| Unit of Measure | 5 mg |
| Product Group | ATTO 386 H |
| Product Type | Hydrophilic Fluorescent Label |
| Fluorescence Maximum (nm) | 472 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.45 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.08 |
| Longest Wavelength Absorption Maximum (nm) | 386 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 25000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 5 |