ATTO Thio12 NHS-ester Fluorescent Label 1 mg
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Product No. AD-THIO12-31
ATTO Thio12 is a new label closely related to the well-known rhodamines.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 582 nm
- ε max = 1.1×105 M -1 cm -1
- λ fl = 607 nm
- n fl = 15 %
- τ fl = 2.0 ns
- CF 260 = ε 260 /ε max = 0.11
- CF 280 = ε 280 / ε max = 0.37
ATTO Thio12 is a new label closely related to the well-known rhodamines. The characteristic feature of the label is high yield of triplet formation. The dye is moderately hydrophilic. After coupling to a substrate the label carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 560 - 590 nm.
| Modification | NHS-ester |
| Unit of Measure | 1 mg |
| Product Group | ATTO Thio12 |
| Product Type | Fluorescent Label |
| Fluorescence Maximum (nm) | 607 |
| MH+ (g/mol) | 599 |
| Molecular Weight (g/mol) | 699 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.11 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.37 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 484.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 624.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 582 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 110000 |
| Fluorescence Quantum Yield (%) | 15 |
| Real Fluorescence Decay Time (ns) | 2 |
| Triplet Quantum Yield (%) | 20 |