ATTO Rho6G alkyne Fluorescent Label 1 mg
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Product No. AD-RHO6G-141
ATTO Rho6G is a new rhodamine dye, based on the well-known laser dye Rhodamine 6G.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 533 nm
- ε max = 1.15×105 M -1 cm -1
- λ fl = 557 nm
- n fl = 94 %
- τ fl = 4.1 ns
- CF 260 = ε 260 /ε max = 0.19
- CF 280 = ε 280 / ε max = 0.16
ATTO Rho6G is a new rhodamine dye, based on the well-known laser dye Rhodamine 6G. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. After coupling to a substrate ATTO Rho6G carries a net electric charge of +1. The fluorescence is excited efficiently in the range 515 - 545 nm.
A suitable excitation source for ATTO Rho6G is the 532 nm line of the frequency-doubled Nd:YAG laser.
| Modification | Alkyne |
| Unit of Measure | 1 mg |
| Product Group | ATTO Rho6G |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 532 |
| Fluorescence Maximum (nm) | 557 |
| MH+ (g/mol) | 551 |
| Molecular Weight (g/mol) | 651 |
| Light Source | Nd:YAG laser, frequency doubled |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.19 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.16 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 496.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 636.7 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 533 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 115000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 4.1 |