ATTO Rho3B Biotin Fluorescent Label 1 mg
$undefined
Product No. AD-RHO3B-71
ATTO Rho3B is a new fluorescent label related to the well-known dye Rhodamine B.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 566 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 589 nm
- n fl = 50 %
- τ fl = 1.5 ns
- CF 260 = ε 260 /ε max = 0.27
- CF 280 = ε 280 / ε max = 0.13
ATTO Rho3B is a new fluorescent label related to the well-known dye Rhodamine B. The dye shows strong absorption and high thermal and photo-stability. The characteristic feature of the dye is its temperature depending fluorescence yield. The dye is moderately hydrophilic. ATTO Rho3B is a cationic dye.
After coupling to a substrate the dye carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 545 - 575 nm.
| Modification | Biotin |
| Unit of Measure | 1 mg |
| Product Group | ATTO Rho3B |
| Product Type | Fluorescent Label |
| Fluorescence Maximum (nm) | 589 |
| MH+ (g/mol) | 852 |
| Molecular Weight (g/mol) | 966 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.27 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.13 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 524.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 664.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 566 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 50 |
| Real Fluorescence Decay Time (ns) | 1.5 |