ATTO Rho14 Biotin Fluorescent Label 5 mg
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Product No. AD-RHO14-75
ATTO Rho14 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA or proteins.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 625 nm
- ε max = 1.4×105 M -1 cm -1
- λ fl = 646 nm
- n fl = 80 %
- τ fl = 3.7 ns
- CF 260 = ε 260 /ε max = 0.29
- CF 280 = ε 280 / ε max = 0.46
ATTO Rho14 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA or proteins. The label shows strong absorption and extraordinarily high fluorescence quantum yield. In fact ATTO Rho14 is the brightest label available in this wavelength range. In addition the dye exhibits an exceptionally high photo-stability. In common with most ATTO-labels, absorption and fluorescence are pH-independent in the range of pH 2 to 11, used in typical applications.
The dye is moderately hydrophilic. After coupling to a substrate ATTO Rho14 carries a net electrical charge of +1. The fluorescence is excited efficiently in the range 605 - 640 nm. A suitable excitation source for ATTO Rho14 is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm.
| Modification | Biotin |
| Unit of Measure | 5 mg |
| Product Group | ATTO Rho14 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 577 |
| Fluorescence Maximum (nm) | 646 |
| MH+ (g/mol) | 1094 |
| Molecular Weight (g/mol) | 1194 |
| Light Source | Mercury arc lamp |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.26 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.47 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 766.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 906.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 626 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 140000 |
| Fluorescence Quantum Yield (%) | 80 |
| Real Fluorescence Decay Time (ns) | 3.7 |