ATTO Rho13 NHS-ester Fluorescent Label 1 mg
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Product No. AD-RHO13-31
ATTO Rho13 is a new fluorescent label belonging to the class of rhodamines.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 603 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 627 nm
- n fl = 80 %
- τ fl = 3.9 ns
- CF 260 = ε 260 /ε max = 0.28
- CF 280 = ε 280 / ε max = 0.43
ATTO Rho13 is a new fluorescent label belonging to the class of rhodamines. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. ATTO Rho13 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
The label is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 545 - 575 nm.
| Modification | NHS-ester |
| Unit of Measure | 1 mg |
| Product Group | ATTO Rho13 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 577 |
| Fluorescence Maximum (nm) | 627 |
| MH+ (g/mol) | 743 |
| Molecular Weight (g/mol) | 843 |
| Light Source | Mercury arc lamp |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.28 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.43 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 628.8 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 769.0 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 603 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 80 |
| Real Fluorescence Decay Time (ns) | 3.9 |
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