ATTO Rho11 Carboxy Fluorescent Label 1 mg
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Product No. AD-RHO11-21
ATTO Rho11 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA, proteins etc.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
ATTO Rho11 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA, proteins etc. The label shows very high fluorescence efficiency and exceptionally high photo-stability.
The dye is moderately hydrophilic. ATTO Rho11 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 550 - 580 nm.
| Modification | Carboxy |
| Unit of Measure | 1 mg |
| Product Group | ATTO Rho11 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 532 |
| Fluorescence Maximum (nm) | 595 |
| MH+ (g/mol) | 566 |
| Molecular Weight (g/mol) | 666 |
| Light Source | Nd:YAG laser, frequency doubled |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.26 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.10 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 548.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 688.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 572 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 80 |
| Real Fluorescence Decay Time (ns) | 4 |