ATTO Oxa12 Biotin Fluorescent Label 1 mg
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Product No. AD-OXA12-71
ATTO Oxa12 is a lipophilic variety of ATTO 655.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 662 nm
- ε max = 1.25×105 M -1 cm -1
- λ fl = 681 nm
- n fl = 30 %
- τ fl = 1.8 ns
- CF 260 = ε 260 /ε max = 0.32
- CF 280 = ε 280 / ε max = 0.12
ATTO Oxa12 is a lipophilic variety of ATTO 655.
Characteristic features of the label are strong absorption, good fluorescence quantum yield, excellent thermal and photo-stability, and outstanding ozone resistance. Absorption and fluorescence are pH-independent in the range pH 2 to 11, used in typical applications.
ATTO Oxa12 shows excellent solubility in polar organic solvents like DMF, DMSO or acetonitrile. ATTO Oxa12 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
| Modification | Biotin |
| Unit of Measure | 1 mg |
| Product Group | ATTO Oxa12 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 647, 676 |
| Fluorescence Maximum (nm) | 681 |
| MH+ (g/mol) | 949 |
| Molecular Weight (g/mol) | 1063 |
| Light Source | Krypton-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.32 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.12 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 621.9 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 762.0 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 662 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 125000 |
| Fluorescence Quantum Yield (%) | 30 |
| Real Fluorescence Decay Time (ns) | 1.8 |