ATTO MB2 Alkyne Redox Label 5 mg
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Product No. AD-MB2-145
ATTO MB2 is a derivative of the well-known redox dye Methylene Blue.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 668 nm
- ε max = 1.1×105 M -1 cm -1
- CF 260 = ε 260 /ε max = 0.08
- CF 2800 = ε 280 /ε max = 0.24
ATTO MB2 is a derivative of the well-known redox dye Methylene Blue. The dye can be reversibly reduced to the colorless leuko form. Upon oxidation (e.g. with oxygen) the dye recovers and the absorption is fully restored. In common with most ATTO-labels the dye shows a high extinction coefficient.
The dye is moderately hydrophilic. ATTO MB2 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
- High thermal and photo-stability
- Redox Label
- Moderately hydrophilic
- Cationic dye
| Modification | Alkyne |
| Unit of Measure | 5 mg |
| Product Group | ATTO MB2 |
| Product Type | Redox Label |
| MH+ (g/mol) | 393 |
| Molecular Weight (g/mol) | 507 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.08 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.24 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 338.4 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 478.5 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 668 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 110000 |