ATTO Cyan 500-3 NHS-ester Fluorescent Label 5 mg
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Product No. AD-CYAN500-35
Cyan 500 NHS-Ester is an amine-reactive fluorescent label.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical Properties (in PBS, pH 7.4)
- λabs = 450nm
- εmax = 4.5×104M-1 cm-1
- λfl = 495nm
- ηfl = 85%
- CF260 = ε260/εmax = 0.31
- CF280 = ε280/εmax = 0.30
Cyan 500 NHS-Ester is an amine-reactive fluorescent label. It is designed and recommended by Roche for application in LightCycler® instruments. The dye is used to label amino-modified oligonucleotides which can be detected on excitation at 430 nm in Roche LightCycler® instruments.
Cyan 500 NHS-Ester can also be used as label for other biomolecules such as proteins etc. The dye has a molecular weight of 580 g/mol and is moderately hydrophilic.
| Modification | NHS-Ester |
| Unit of Measure | 5 mg |
| Product Group | ATTO CYAN |
| Product Type | Fluorescent Label |
| Fluorescence Maximum (nm) | 495 |
| MH+ (g/mol) | 581 |
| Molecular Weight (g/mol) | 580 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.31 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.3 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 450 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 45000 |
| Fluorescence Quantum Yield (%) | 85 |