ATTO 740 Maleimide Fluorescent Label 1 mg
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Product No. AD-740-41
ATTO 740 together with ATTO 725 belongs to a new generation of fluorescent labels for the near infrared spectral region.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 743 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 763 nm
- n fl = 10 %
- τ fl = 0.6 ns
- CF 260 = ε 260 /ε max = 0.07
- CF 2800 = ε 280 /ε max = 0.07
ATTO 740 together with ATTO 725 belongs to a new generation of fluorescent labels for the near infrared spectral region.
Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. ATTO 740 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction. The fluorescence is excited most efficiently in the range 720 - 755 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Maleimide |
| Unit of Measure | 1 mg |
| Product Group | ATTO 740 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 647, 676 |
| Fluorescence Maximum (nm) | 763 |
| MH+ (g/mol) | 590 |
| Molecular Weight (g/mol) | 690 |
| Light Source | Krypton-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.07 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.07 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 450.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 590.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 743 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 10 |
| Real Fluorescence Decay Time (ns) | 0.6 |