ATTO 725 NHS-ester Fluorescent Label 5 mg
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Product No. AD-725-35
ATTO 725 together with ATTO 740 belongs to a new generation of fluorescent labels for the near infrared spectral region.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 728 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 751 nm
- n fl = 10 %
- τ fl = 0.5 ns
- CF 260 = ε 260 /ε max = 0.08
- CF 2800 = ε 280 /ε max = 0.06
ATTO 725 together with ATTO 740 belongs to a new generation of fluorescent labels for the near infrared spectral region.
Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. ATTO 725 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 725 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction. The fluorescence is excited most efficiently in the range 700 - 745 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | NHS-ester |
| Unit of Measure | 5 mg |
| Product Group | ATTO 725 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 647, 676 |
| Fluorescence Maximum (nm) | 751 |
| MH+ (g/mol) | 513 |
| Molecular Weight (g/mol) | 613 |
| Light Source | Krypton-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.08 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.06 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 398.5 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 538.7 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 728 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 10 |
| Real Fluorescence Decay Time (ns) | 0.5 |