ATTO 700 Carboxy Fluorescent Label 5 mg
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Product No. AD-700-25
ATTO 700 belongs together with ATTO 655 and ATTO 680 to a new generation of fluorescent labels.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 700 nm
- ε max = 1.25×105 M -1 cm -1
- λ fl = 716 nm
- n fl = 25 %
- τ fl = 1.6 ns
- CF 260 = ε 260 /ε max = 0.26
- CF 2800 = ε 280 /ε max = 0.41
ATTO 700 belongs together with ATTO 655 and ATTO 680 to a new generation of fluorescent labels.
Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 700 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral.
ATTO 700 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 670 - 715 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Carboxy |
| Unit of Measure | 5 mg |
| Product Group | ATTO 700 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 647, 676 |
| Fluorescence Maximum (nm) | 716 |
| MH+ (g/mol) | 566 |
| Molecular Weight (g/mol) | 679 |
| Light Source | Krypton-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.26 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.41 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 547.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 687.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | 0 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 700 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 25 |
| Real Fluorescence Decay Time (ns) | 1.6 |