ATTO 680 Iodoacetamide Fluorescent Label 5 mg
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Product No. AD-680-115
ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 681 nm
- ε max = 1.25×105 M -1 cm -1
- λ fl = 698 nm
- n fl = 30 %
- τ fl = 1.7 ns
- CF 260 = ε 260 /ε max = 0.30
- CF 280 = ε 280 / ε max = 0.17
ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.
Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 680 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 680 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
The fluorescence is excited most efficiently in the range 645 - 695 nm. A suitable excitation source for the dye is the Krypton-Ion laser using the 676 nm line or a diode-laser emitting at 670 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Iodoacetamide |
| Unit of Measure | 5 mg |
| Product Group | ATTO 680 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 647, 676 |
| Fluorescence Maximum (nm) | 698 |
| MH+ (g/mol) | 736 |
| Molecular Weight (g/mol) | 850 |
| Light Source | Krypton-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.30 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.17 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 507.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 647.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | 0 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 681 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 125000 |
| Fluorescence Quantum Yield (%) | 30 |
| Real Fluorescence Decay Time (ns) | 1.7 |