ATTO 655 Amine Fluorescent Label 1 mg
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Product No. AD-655-91
ATTO 655 belongs together with ATTO 680 and ATTO 700 to a new generation of fluorescent labels.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 663 nm
- ε max = 1.25×105 M -1 cm -1
- λ fl = 680 nm
- n fl = 30 %
- τ fl = 1.8 ns
- CF 260 = ε 260 /ε max = 0.24
- CF 280 = ε 280 / ε max = 0.08
ATTO 655 belongs together with ATTO 680 and ATTO 700 to a new generation of fluorescent labels.
Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, exceptionally high stability towards atmospheric ozone, and very good water solubility. Thus ATTO 655 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
ATTO 655 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 655 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 640 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Amine |
| Unit of Measure | 1 mg |
| Product Group | ATTO 655 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 633, 647, 676, 635 |
| Fluorescence Maximum (nm) | 680 |
| MH+ (g/mol) | 570 |
| Molecular Weight (g/mol) | 798 |
| Light Source | He-Ne laser, Krypton-Ion laser, Diode laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.24 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.08 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 509.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 649.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | 0 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 663 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 125000 |
| Fluorescence Quantum Yield (%) | 30 |
| Real Fluorescence Decay Time (ns) | 1.8 |