ATTO 647N Tetrazine (MeTet) Fluorescent Label 5 mg
$undefined
Product No. AD-647N-2505
ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 646 nm
- ε max = 1.5×105 M -1 cm -1
- λ fl = 664 nm
- n fl = 65 %
- τ fl = 3.5 ns
- CF 260 = ε 260 /ε max = 0.04
- CF 280 = ε 280 / ε max = 0.03
ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. Thus ATTO 647N is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic. ATTO 647N is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
As supplied ATTO 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Tetrazine (MeTet) |
| Unit of Measure | 0.5 mg |
| Product Group | ATTO 647N |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 633, 647, 676, 635 |
| Fluorescence Maximum (nm) | 664 |
| MH+ (g/mol) | 830 |
| Molecular Weight (g/mol) | 930 |
| Light Source | He-Ne laser, Krypton-Ion laser, Diode laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.04 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.03 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 628.9 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 769.0 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 646 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 150000 |
| Fluorescence Quantum Yield (%) | 65 |
| Real Fluorescence Decay Time (ns) | 3.5 |