ATTO 647 Streptavidin Fluorescent Label 1 mg
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Product No. AD-647-61
ATTO 647 belongs to a new generation of fluorescent labels for the red spectral region.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 647 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 667 nm
- n fl = 20 %
- τ fl = 2.4 ns
- CF 260 = ε 260 /ε max = 0.08
- CF 280 = ε 280 / ε max = 0.04
ATTO 647belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and good water solubility. ATTO 647 is a zwitterionic dye with a net electrical charge of zero. The dye is stable at physiological pH-values and in buffers up to pH 8. However, it slowly degrades at higher pH. The dye is very hydrophilic.
The fluorescence is excited most efficiently in the range 615 - 660 nm. A suitable excitation source for ATTO 647 is, next to the He:Ne laser (633nm), the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Streptavidin |
| Unit of Measure | 1 mg |
| Product Group | ATTO 647 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 633, 647, 676, 635 |
| Fluorescence Maximum (nm) | 667 |
| Light Source | He-Ne laser, Krypton-Ion laser, Diode laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.08 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.04 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 574.8 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 714.9 |
| Change of Electrical Charge on Conjugation with ATTO-Label | 0 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 647 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 20 |
| Real Fluorescence Decay Time (ns) | 2.4 |