ATTO 643 Peg(3)-Azide Fluorescent Label 1 mg
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Product No. AD-643-101
ATTO 643 is a new fluorescent label related to the well known ATTO 647N.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 643 nm
- ε max = 1.5×105 M -1 cm -1
- λ fl = 655 nm
- n fl = 62 %
- τ fl = 3.5 ns
- CF 260 = ε 260 /ε max = 0.05
- CF 280 = ε 280 / ε max = 0.04
ATTO 643 is a new fluorescent label related to the well known ATTO 647N. Characteristic features of the label are strong absorption and high thermal and photo-stability. The dye is very hydrophilic, hence it shows excellent water solubility and significantly reduced tendency for unspecific binding. ATTO 643 exhibits an extraordinary high fluorescence quantum yield. Contrary to many common fluorescent labels this is the case even when the dye is coupled to proteins, e.g. antibodies, streptavidin etc.
The dye is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. The label is also highly recommended for oligonucleotide labeling, flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 643 is an anionic dye. After coupling to a substrate the dye carries a net electrical charge of -1. As supplied ATTO 643 consists of a mixture of three isomers with practically identical absorption and fluorescence properties. The fluorescence is excited most efficiently in the range 625 - 660 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Peg(3)-Azide |
| Unit of Measure | 1 mg |
| Product Group | ATTO 643 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 633, 635 |
| Fluorescence Maximum (nm) | 665 |
| MH+ (g/mol) | 1036 |
| Molecular Weight (g/mol) | 1058 |
| Light Source | He-Ne laser, Diode laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.05 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.04 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 814.0 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 953.2 |
| Change of Electrical Charge on Conjugation with ATTO-Label | -1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 643 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 150000 |
| Fluorescence Quantum Yield (%) | 62 |
| Real Fluorescence Decay Time (ns) | 3.5 |