ATTO 633 Alkyne Fluorescent Label 5 mg

Optical properties

• λ _abs = 630 nm
• ε _max = 1.3×10⁵ M ^-1 cm ^-1
• λ _fl = 65 1nm
• n _fl = 64 %
• τ _fl = 3.3 ns
• CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.04
• CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.05

ATTO 633 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 633 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 633 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1.

The label is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 610 - 645 nm. A suitable excitation source for ATTO 633 is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm.