ATTO 633 Alkyne Fluorescent Label 5 mg
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Product No. AD-633-145
ATTO 633 belongs to a new generation of fluorescent labels for the red spectral region.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 630 nm
- ε max = 1.3×105 M -1 cm -1
- λ fl = 65 1nm
- n fl = 64 %
- τ fl = 3.3 ns
- CF 260 = ε 260 /ε max = 0.04
- CF 280 = ε 280 / ε max = 0.05
ATTO 633 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 633 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 633 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1.
The label is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 610 - 645 nm. A suitable excitation source for ATTO 633 is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Alkyne |
| Unit of Measure | 5 mg |
| Product Group | ATTO 633 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 633, 647, 676, 635 |
| Fluorescence Maximum (nm) | 651 |
| MH+ (g/mol) | 589 |
| Molecular Weight (g/mol) | 703 |
| Light Source | He-Ne laser, Krypton-Ion laser, Diode laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.04 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.05 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 534.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 674.9 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 630 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 130000 |
| Fluorescence Quantum Yield (%) | 64 |
| Real Fluorescence Decay Time (ns) | 3.3 |