ATTO 620 Peg(3)-Azide Fluorescent Label 5 mg
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Product No. AD-620-105
ATTO 620 belongs to a new generation of fluorescent labels for the red spectral region.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 620 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 642 nm
- n fl = 50 %
- τ fl = 2.9 ns
- CF 260 = ε 260 /ε max = 0.04
- CF 280 = ε 280 / ε max = 0.06
ATTO 620 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, temperature dependent fluorescence, and high thermal and photo-stability.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 620 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1
The label is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 600 - 630 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Peg(3)-Azide |
| Unit of Measure | 5 mg |
| Product Group | ATTO 620 |
| Product Type | Fluorescent Label |
| Fluorescence Maximum (nm) | 642 |
| MH+ (g/mol) | 712 |
| Molecular Weight (g/mol) | 812 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.04 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.06 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 494.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 634.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 620 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 50 |
| Real Fluorescence Decay Time (ns) | 2.9 |