ATTO 580Q Maleimide Fluorescence Quencher 1 mg
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Product No. AD-580Q-41
ATTO 580Q is a novel fluorescence quencher (energy acceptor in FRET process).
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 587 nm
- ε max = 1.10×105 M -1 cm -1
- CF 260 = ε 260 /ε max = 0.32
- CF 2800 = ε 280 /ε max = 0.11
ATTO 580Q is a novel fluorescence quencher (energy acceptor in FRET process). Characteristic features of the label are strong absorption and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO 580Q is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 580Q consists of six isomers with practically identical absorption.
- High thermal and photo-stability
- Moderately hydrophilic
- Cationic dye related to rhodamines
- Supplied as mixture of six isomers
| Modification | Maleimide |
| Unit of Measure | 1 mg |
| Product Group | ATTO 580Q |
| Product Type | Fluorescence Quencher |
| MH+ (g/mol) | 817 |
| Molecular Weight (g/mol) | 917 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.32 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.11 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 677.9 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 818.0 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescence Quenchers |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 587 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 110000 |
| Quenching Range (ns) | 535 - 610 |
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