ATTO 565 Carboxy Fluorescent Label 5 mg

Optical properties

• λ _abs = 564 nm
• ε _max = 1.2×10⁵ M ^-1 cm ^-1
• λ _fl = 590 nm
• n _fl = 90 %
• τ _fl = 4.0 ns
• CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.27
• CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.12

ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545 - 575 nm. As supplied ATTO 565 consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on request the dye can also be supplied as single isomer.