ATTO 565 Alkyne Fluorescent Label 5 mg
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Product No. AD-565-145
ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 564 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 590 nm
- n fl = 90 %
- τ fl = 4.0 ns
- CF 260 = ε 260 /ε max = 0.27
- CF 280 = ε 280 / ε max = 0.12
ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545 - 575 nm. As supplied ATTO 565 consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on request the dye can also be supplied as single isomer.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Alkyne |
| Unit of Measure | 5 mg |
| Product Group | ATTO 565 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 546, 532 |
| Fluorescence Maximum (nm) | 590 |
| MH+ (g/mol) | 548 |
| Molecular Weight (g/mol) | 648 |
| Light Source | Mercury arc lamp, Nd:YAG laser, frequency doubled |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.27 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.12 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 492.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 632.7 |
| Change of Electrical Charge on Conjugation with ATTO-Label | 0 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 564 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 4 |