ATTO 550 Alkyne Fluorescent Label 1 mg
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Product No. AD-550-141
ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 554 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 576 nm
- n fl = 80 %
- τ fl = 3.6 ns
- CF 260 = ε 260 /ε max = 0.23
- CF 280 = ε 280 / ε max = 0.10
ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability.
The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence.
The fluorescence is excited most efficiently in the range 540 - 565 nm.
| Modification | Alkyne |
| Unit of Measure | 1 mg |
| Product Group | ATTO 550 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 546, 532 |
| Fluorescence Maximum (nm) | 576 |
| MH+ (g/mol) | 631 |
| Molecular Weight (g/mol) | 731 |
| Light Source | Mercury arc lamp, Nd:YAG laser, frequency doubled |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.23 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.10 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 576.8 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 716.9 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Longest Wavelength Absorption Maximum (nm) | 554 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 80 |
| Real Fluorescence Decay Time (ns) | 3.6 |