ATTO 542 Peg(3)-Azide Fluorescent Label 1 mg
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Product No. AD-542-101
ATTO 542 is a new fluorescent label related to ATTO 532 .
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 542 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 562 nm
- n fl = 93 %
- τ fl = 3.7 ns
- CF 260 = ε 260 /ε max = 0.18
- CF 280 = ε 280 / ε max = 0.08
ATTO 542 is a new fluorescent label related to ATTO 532.
The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
After coupling to a substrate the dye carries a net electrical charge of - 3. The fluorescence is excited most efficiently in the range 520 - 555 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Peg(3)-Azide |
| Unit of Measure | 1 mg |
| Product Group | ATTO 542 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 514, 532 |
| Fluorescence Maximum (nm) | 562 |
| MH+ (g/mol) | 1114 |
| Molecular Weight (g/mol) | 1228 |
| Light Source | Argon-Ion laser, Nd:YAG laser, frequency doubled |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.18 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.08 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 893.0 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 1033.1 |
| Change of Electrical Charge on Conjugation with ATTO-Label | -3 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 542 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 120000 |
| Fluorescence Quantum Yield (%) | 93 |
| Real Fluorescence Decay Time (ns) | 3.7 |