ATTO 540Q Biotin Fluorescence Quencher 1 mg
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Product No. AD-540Q-71
ATTO 540Q is a novel fluorescence quencher (energy acceptor in FRET process).
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 543 nm
- ε max = 1.05×105 M -1 cm -1
- CF 260 = ε 260 /ε max = 0.26
- CF 2800 = ε 280 /ε max = 0.27
ATTO 540Q is a novel fluorescence quencher (energy acceptor in FRET process). Characteristic features of the label are strong absorption and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO 540Q is a cationic dye.
After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 540Q consists of a mixture of two isomers with practically identical absorption.
- High thermal and photo-stability
- Moderately hydrophilic
- Cationic rhodamine dye
| Modification | Biotin |
| Unit of Measure | 1 mg |
| Product Group | ATTO 540Q |
| Product Type | Fluorescence Quencher |
| MH+ (g/mol) | 869 |
| Molecular Weight (g/mol) | 970 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.27 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.26 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 541.6 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 681.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescence Quenchers |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 543 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 105000 |
| Quenching Range (ns) | 500 - 565 |
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