ATTO 532 NHS-ester Fluorescent Label 5 mg
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Product No. AD-532-35
ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 532 nm
- ε max = 1.15×105 M -1 cm -1
- λ fl = 552 nm
- n fl = 90 %
- τ fl = 3.8 ns
- CF 260 = ε 260 /ε max = 0.20
- CF 280 = ε 280 / ε max = 0.09
ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and excellent water solubility. Thus ATTO 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
The fluorescence is excited most efficiently in the range 515 - 545 nm. A suitable excitation source for ATTO 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | NHS-ester |
| Unit of Measure | 5 mg |
| Product Group | ATTO 532 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 514, 532 |
| Fluorescence Maximum (nm) | 552 |
| MH+ (g/mol) | 743 |
| Molecular Weight (g/mol) | 1081 |
| Light Source | Argon-Ion laser, Nd:YAG laser, frequency doubled |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.20 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.09 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 626.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 766.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | - 1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 532 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 115000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 3.8 |