ATTO 520 NHS-ester Fluorescent Label 5 mg
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Product No. AD-520-35
ATTO 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 517 nm
- ε max = 1.1×105 M -1 cm -1
- λ fl = 538 nm
- n fl = 90 %
- τ fl = 3.6 ns
- CF 260 = ε 260 /ε max = 0.16
- CF 280 = ε 280 / ε max = 0.20
ATTO 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. Above pH 7.0 the dye exists in an equilibrium with a colorless form (pseudobase). This is a reversible reaction, which does not interfere with the process of coupling to proteins etc. On lowering the pH absorption and fluorescence are restored fully. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | NHS-ester |
| Unit of Measure | 5 mg |
| Product Group | ATTO 520 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 488, 514 |
| Fluorescence Maximum (nm) | 538 |
| MH+ (g/mol) | 464 |
| Molecular Weight (g/mol) | 564 |
| Light Source | Argon-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.16 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.20 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 349.5 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 489.6 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 517 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 110000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 3.6 |