ATTO 520 DMPE Labeled Phospholipid 1 mg
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Product No. AD-520-191
ATTO-Dye Labeled Phospholipids
In recent years the investigation of biological membranes, e.g. intracellular membranes of live cells, plasma membranes etc., has become a major subject of interest. As a result there is a growing demand for fluorescent dye-labeled lipids, in particular glycero-phospholipids, to be incorporated in biological membranes. ATTO offers a variety of glycero-phospholipids, carrying one or two fatty acid chains (lipophilic groups) and a phosphate ester residue (hydrophilic group).
ATTO-dye fluorescent phospholipids are labeled at the hydrophilic head group. After incorporation in, e.g., a plasma membrane (phospholipid bilayer) the fluorophore is located at the water/lipid interface of the membrane.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Currently we provide the phospholipids
- 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE),
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE),
- 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (PPE)
- 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), and
- 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE)
labeled with ATTO-dyes.
- High fluorescence yield
- High thermal and photo-stability
- Hydrophobic
- At pH > 7 reversible formation of colorless pseudobase
- Cationic dye closely related to well-known Rhodamine 6G
| Modification | DMPE |
| Unit of Measure | 1 mg |
| Product Group | ATTO 520 |
| Product Type | Labeled Phospholipid |
| Emission Line (nm) | 488, 514 |
| Fluorescence Maximum (nm) | 538 |
| Light Source | Argon-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.16 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.20 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 349.5 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 489.6 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 517 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 110000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 3.6 |