ATTO 490 LS Phalloidin LS-Dye Series 10 nmol
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Product No. AD-490LS-81
ATTO 490LS is a new fluorescent marker with an exceptionally large Stokes shift of 165 nm.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 496 nm
- ε max = 4.0×104 M -1 cm -1
- λ fl = 661 nm
- n fl = 30 %
- τ fl = 2.6 ns
- CF 260 = ε 260 /ε max = 0.37
- CF 2800 = ε 280 /ε max = 0.18
ATTO 490LS is a new fluorescent label featuring an extraordinary large Stokes-shift of 165 nm. Thus the emission spectrum is almost completely separated from the absorption spectrum, making the dye highly suitable for multicolor experiments, in particular in combination with ATTO 488 and ATTO 514.
ATTO 490LS is very hydrophilic, highly water soluble, and exhibits a relatively high fluorescence quantum yield, which is only slightly reduced on conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL).
ATTO 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1. The fluorescence is excited most efficiently in the range 460 - 530 nm.
- Extraordinary large Stokes-shift
- High fluorescence yield
- Highly fluorescent even after conjugation
- Very hydrophilic
- Excellent water solubility
- Very little aggregation
- Highly suitable for single-molecule applications and high-resolution microscopy
| Modification | Phalloidin |
| Unit of Measure | 10 nmol |
| Product Group | ATTO 490 LS |
| Product Type | LS-Dye Series |
| Emission Line (nm) | 488, 514 |
| Fluorescence Maximum (nm) | 658 |
| Light Source | Argon-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.39 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.21 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 654.8 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 795.0 |
| Change of Electrical Charge on Conjugation with ATTO-Label | - 1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 495 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 40000 |
| Fluorescence Quantum Yield (%) | 30 |
| Real Fluorescence Decay Time (ns) | 2.6 |