ATTO 488 Biotin Fluorescent Label 5 mg

Optical properties

λ _abs = 500 nm

ε _max = 9.0×10⁴ M ^-1 cm ^-1

λ _fl = 520 nm

n _fl = 80 %

τ _fl = 4.1 ns

CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.22

CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.09

ATTO 488 is a hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield, and exceptional thermal and photo-stability. Thus ATTO 488 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more. The fluorescence is excited most efficiently in the range 480 - 515 nm.

A suitable source of excitation is the 488 nm line of an Argon-Ion laser.