ATTO 465 Maleimide Fluorescent Label 1 mg
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Product No. AD-465-41
ATTO 465 is a novel fluorescent label derived from the well-known dye Acriflavin. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 453 nm
- ε max = 7.5×104 M -1 cm -1
- λ fl = 506 nm
- n fl = 70 %
- τ fl = 5.0 ns
- CF 260 = ε 260 /ε max = 1.09
- CF 280 = ε 280 / ε max = 0.48
ATTO 465 is a novel fluorescent label derived from the well-known dye Acriflavin. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. In aqueous solution the dye shows a surprisingly large Stokes-shift of 55 nm. The dye shows intense and long-lived phosphorescence in solid matrix, in particular at low temperatures. ATTO 465 is moderately hydrophilic. The fluorescence can be excited efficiently in the range 420 - 465 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Maleimide |
| Unit of Measure | 1 mg |
| Product Group | ATTO 465 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 436 |
| Fluorescence Maximum (nm) | 506 |
| MH+ (g/mol) | 418 |
| Molecular Weight (g/mol) | 518 |
| Light Source | Mercury arc lamp |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 1.09 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.48 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 278.4 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 418.5 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 453 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 75000 |
| Fluorescence Quantum Yield (%) | 75 |
| Real Fluorescence Decay Time (ns) | 5 |
| Triplet Quantum Yield (%) | 10 |