ATTO 430 LS Azide LS-Dye Series 5 mg
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Product No. AD-430LS-105
ATTO 430LS is a novel fluorescent marker with an exceptionally large Stokes shift of 109 nm. Thus, emission is almost completely spectrally separated from absorption.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 436 nm
- ε max = 3.2×104 M -1 cm -1
- λ fl = 545 nm
- n fl = 65 %
- τ fl = 4.0 ns
- CF 260 = ε 260 /ε max = 0.32
- CF 2800 = ε 280 /ε max = 0.32
ATTO 430LS is a new fluorescent label featuring an extraordinary large Stokes-shift of 109 nm. Thus the emission spectrum is almost completely separated from the absorption spectrum. ATTO 430LS is very hydrophilic and highly water soluble. The dye exhibits a high fluorescence quantum yield, which is only slightly reduced on conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL).
ATTO 430LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1. The fluorescence is excited most efficiently in the range 400 - 460 nm.
- Extraordinary large Stokes-shift
- High fluorescence yield
- Highly fluorescent even after conjugation
- Very hydrophilic
- Excellent water solubility
- Very little aggregation
- Highly suitable for single-molecule applications and high-resolution microscopy
| Modification | Azide |
| Unit of Measure | 5 mg |
| Product Group | ATTO 430 LS |
| Product Type | LS-Dye Series |
| Emission Line (nm) | 405, 436 |
| Fluorescence Maximum (nm) | 545 |
| Light Source | Mercury arc lamp |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.32 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.22 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 547.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 687.8 |
| Change of Electrical Charge on Conjugation with ATTO-Label | - 1 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 436 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 32000 |
| Fluorescence Quantum Yield (%) | 65 |
| Real Fluorescence Decay Time (ns) | 4 |