ATTO 425 Carboxy Fluorescent Label 5 mg
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Product No. AD-425-25
ATTO 425 is a novel fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, high photo-stability, and low molecular weight.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 439 nm
- ε max = 4.5×104 M -1 cm -1
- λ fl = 485 nm
- n fl = 90 %
- τ fl = 3.6 ns
- CF 260 = ε 260 /ε max = 0.19
- CF 280 = ε 280 / ε max = 0.17
ATTO 425 is a novel fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, high photo-stability, and low molecular weight. ATTO 425 is moderately hydrophilic. Its fluorescence can be excited efficiently in the range 405 - 455 nm.
Characteristic properties of our fluorescent dyes
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
| Modification | Carboxy |
| Unit of Measure | 5 mg |
| Product Group | ATTO 425 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 405, 436 |
| Fluorescence Maximum (nm) | 485 |
| MH+ (g/mol) | 402 |
| Molecular Weight (g/mol) | 401 |
| Light Source | Mercury arc lamp |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.19 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.17 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 383.4 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 523.6 |
| Change of Electrical Charge on Conjugation with ATTO-Label | 0 |
| Microscope Group | Fluorescent Labels |
| Applications | Fluorescence |
| Longest Wavelength Absorption Maximum (nm) | 439 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 45000 |
| Fluorescence Quantum Yield (%) | 90 |
| Real Fluorescence Decay Time (ns) | 3.6 |