Functional Studies - Iron Assay Kit (Colorimetric) (ab83366)

Leal SM Jr et al examined if iron availability regulates fungal growth in an infection as fungal infection intiates an iron sequestration response. Mice given Fe-dextran (Fe-Dex) and deferoxamine (Defox) shows a higher fungal mass (Fungal dsRed) compared to vehicle treated mice over 48 hours. Iron content was quantified in mouse serum using Iron assay kit (ab83366).

Leal SM Jr et al., PLoS Pathog 9(7), fig 2c. doi: 10.1371/journal.ppat.1003436. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Iron Assay Kit (Colorimetric) (ab83366)

Iron measured in human urine showing concentration (micromolar)

Functional Studies - Iron Assay Kit (Colorimetric) (ab83366)

Assay of soluble free iron from a soil sample (5 μL of 100 μL buffer into which 100 mg of soil had been stirred), 5 μL of FBS and 5 μL of a 100 μM sample of iron standard.

Functional Studies - Iron Assay Kit (Colorimetric) (ab83366)

Example of iron standard curve using ab83366.

Functional Studies - Iron Assay Kit (Colorimetric) (ab83366)

Assay of total iron(II + III) in off-the-clot human serum (50 μl of serum was added per well). Data are mean ± SEM from 2 independent replicates, performed in duplicate wells.