ELISA - Human ALT ELISA Kit (ab234578)

Example of human ALT standard curve in Sample Diluent NS + Enhancer.

The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ALT ELISA Kit (ab234578)

Example of human ALT standard curve in Sample Diluent NS + 1X Enhancer. The ALT standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ALT ELISA Kit (ab234578)

Interpolated concentrations of native ALT in human serum, plasma (EDTA) and plasma (citrate) samples.

The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 4,002.9 pg/mL in serum, 2,685.5 pg/mL in plasma (EDTA) and 1,886.6 pg/mL in plasma (citrate).

Sandwich ELISA - Human ALT ELISA Kit (ab234578)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native ALT was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human ALT ELISA Kit (ab234578)

Linearity of dilution.

Recombinant ALT was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer.