Sandwich ELISA - mCherry ELISA Kit (ab221829)
Example of mCherry standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - mCherry ELISA Kit (ab221829)
Interpolated concentrations of spiked mCherry in cell culture supernatant samples.
The concentrations of mCherry were measured in duplicates, interpolated from the mCherry standard curves and corrected for sample dilution. Undiluted samples are as follows: RPMI culture media with 10% FBS (100%), and HUVEC supernatant (100%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean mCherry concentration was determined to be 204.81 pg/mL in spiked RPMI culture media with 10% FBS (100%) and 212.94 pg/mL in spiked HUVEC supernatant (100%). HUVEC cells were cultured in RPMI media with 10% FBS and supernatant collected.
Sandwich ELISA - mCherry ELISA Kit (ab221829)
Example of mCherry standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - mCherry ELISA Kit (ab221829)
Interpolated concentrations of spiked mCherry in HEK 293T and 143B (wild-type) cell extract samples based on a 200 μg/mL extract load.
The concentrations of mCherry were measured in duplicate and interpolated from the mCherry standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean mCherry concentration was determined to be 182.08 pg/mL in spiked HEK 293T cell extract and 198.36 pg/mL in spiked 143B cell extract.
Sandwich ELISA - mCherry ELISA Kit (ab221829)
Raw O.D. values are shown for mCherry protein, YFP, GFP, and RFP.
All proteins were loaded at 400 pg/mL. The average background O.D. is represented by the dashed line. Signal from YFP, GFP, and RFP loaded at 400 pg/mL was equivalent to background.
